Synthesis, DNA and Protein Binding and DNA Cleavage Activity of a Ferric (III) Complex Containing Novel TACN

Abstract

A new Ferric(III) complex, [Fe(L)(Cl)3] (1) [L= N-(4-(Benzyloxy)benzyl)-1,4,7-Triazacyclononane], has been synthesized and characterized by IR, elemental analysis and ESI-MS. The electrospray mass spectrum of 1 in solution indicates that mononuclear ion [Fe(L)Cl2]+ (2) is the active species. Fluorescence spectroscopy studies of 2 with calf thymus DNA (ct-DNA) show that 2 partially intercalates to ct-DNA. 2 with bovine serum albumin (BSA) protein investigated using fluorescence method indicated that there occurred strong binding of ferric complex to BSA over the ligand. Further, in the absence of external agents, supercoiled plasmid DNA cleavage by 2 was performed under aerobic condition.