Synthesis, Characterization and Antioxidant Activities of C3, 6-diacetylated Phenyl-thiosemicarbazone-chitosans

Abstract

The purpose of this study was to further optimize the synthesis conditions of different position substituted acetyl phenyl-thiosemicarbazone-chitosans, and assess their antioxidant activities together with cell toxicity. Ten new C3, 6-diacetylated phenyl-thiosemicarbazone-chitosans (with high and low molecular weight) were prepared with located C2-NH2 protecting method for the first time, and their structures were characterized by elemental analysis and FT-IR spectroscopy. In addition, the antioxidant activities of the new derivatives were investigated employing various established systems, such as DPPH scavenging/reducing power/hydroxyl radical (•OH) and superoxide anion (O2 .−). The results indicated that the antioxidant activities of C3, 6-diacetylated phenyl- thiosemicarbazone-chitosans were much better than that of Chitosan. All of the derivatives showed strong antioxidant activities in the investigated concentration range of 25-400 μg·mL-1.