Milk Fat Globule Membrane Proteins in Milk Extraction Conditions Optimization and Enzymolysis Products Antioxidant Activity Research

Abstract

As one of the important composition of milk protein, milk fat globule membrane (MFGM) proteins have many specific physiological functions. By physical method to extract MFGM proteins in milk, the extraction conditions were optimized, and the optimal physical conditions for the extraction of MFGM proteins in milk were determined, amount of sucrose added 4g, and washed 3 times. Using intercept molecular weight 10 ku and 5 ku ultrafiltration membrane carries on the preliminary purification of MFGM antioxidant peptides, respectively measured antioxidant activity, and found that 5ku <molecular weight (MW ） 10ku had higher antioxidant activity, and measured DPPH • clearance, reducing ability. DPPH • clearance, was 70.41%, is the VC 0.8 times; reducing capacity is the VC 0.92 times.