Preparation of a Modified Chitosan and Polyvinyl Alcohol Affinity Membrane and Its Application in Purification of His-Tag Protein

Bu-kun JIN, Rong ZENG, Xiao-yan YIN, Tao Run, Ya-li HOU, Zhong-hua YANG

Abstract


In this work, a modified chitosan and polyvinyl alcohol membranes was prepared and employed for purification of His-Tag protein. First, the membranes were crosslinked with epichlorohydrin and covalently coupled with iminodiacetic acid. Then the Ni2+ was immobilized on membranes by chelation. The application of the affinity membrane for his-tag protein was studied. The effect of salt concentration and initial activity on His-Tag adsorption capacity was investigated. The results showed that the optimal loading conditions were 15℃, 0.6 mol/L NaCl, initial activity 1.0u/ml. The maximum adsorption is 35u/g.

Keywords


Chitosan, Affinity membrane, His-Tag protein


DOI
10.12783/dteees/eccsd2016/5810

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